Development of Immunoassays for Detecting Clothianidin Residue in Agricultural Products

Συγγραφείς:
Li, Ming
Sheng, Enze
Cong, Lujing
Wang, Minghua
Τύπος: Journal Article
'Ετος δημοσίευσης: 2013
Πηγή: USDA
Συλλογή από: AGRIS
Θέμα:
agricultural products
products
Περιγραφή:Two enzyme-linked immunosorbent assays (ELISAs) based on polyclonal antibodies (PcAbs) for clothianidin are described: colorimetric detection format (ELISA) and pattern of chemiluminescent assay (CLEIA). Clothianidin hapten was synthesized and conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to produce immunogen and coating antigen. Anticlothianidin PcAbs were obtained from immunized New Zealand white rabbits. Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC20) of clothianidin were 0.046 and 0.0028 mg/L for the ELISA and 0.015 and 0.0014 mg/L for the CLEIA, respectively. There were no obvious cross-reactivities of the antibodies with its analogues except for dinotefuran. Recoveries of 76.4-116.4% for the immunoassays were achieved from spiked samples. The results of immunoassays for the spiked and authentic samples were largely consistent with gas chromatography. Therefore, the proposed immunoassays would be convenient and satisfactory analytical methods for the monitoring of clothianidin in agricultural products.
 

Σχετικοί Πόροι

Risk Analysis for Off-season Greenhouse Tomato Production

Provider: 
Subject: 
products
agricultural products
plant products
socioeconomic development
agricultural development
economics
agricultural economics
agriculture
agricultural practices

Innovation implementation strategies for consumer driven fruit supply chains

Provider: 
Subject: 
products
agricultural products
socioeconomic development
rural development
agricultural development
economics
agricultural economics
trade
agriculture
agricultural practices

An economic analysis of soilless culture in gerbera production

Provider: 
Subject: 
products
agricultural products
plant products
agriculture
agricultural practices

Thom's Commercial Directory, 1979, Pg.86

Provider: 
Subject: 
agricultural products
products

welfare impact of a free trade agreement between SACU and Mercosur

Provider: 
Subject: 
agricultural products
products
agriculture
trade
farmers
Collected from: 

Steam processed broccoli (Brassica oleracea) has higher antioxidant activity in chemical and cellular assay systems

Provider: 
Subject: 
agricultural products
products
Collected from: 

AGRERI Library

Risk Analysis for Off-season Greenhouse Tomato Production

Provider: 
Subject: 
products
agricultural products
plant products
socioeconomic development
agricultural development
economics
agricultural economics
agriculture
agricultural practices

Innovation implementation strategies for consumer driven fruit supply chains

Provider: 
Subject: 
products
agricultural products
socioeconomic development
rural development
agricultural development
economics
agricultural economics
trade
agriculture
agricultural practices

An economic analysis of soilless culture in gerbera production

Provider: 
Subject: 
products
agricultural products
plant products
agriculture
agricultural practices

External Library

Thom's Commercial Directory, 1979, Pg.86

Provider: 
Subject: 
agricultural products
products

welfare impact of a free trade agreement between SACU and Mercosur

Provider: 
Subject: 
agricultural products
products
agriculture
trade
farmers
Collected from: 

Steam processed broccoli (Brassica oleracea) has higher antioxidant activity in chemical and cellular assay systems

Provider: 
Subject: 
agricultural products
products
Collected from: